| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 541 | Pectobacterium atrosepticum | AGCACGCCAAACCGATCT | AATCACGGGTGAGCCGTT | 59.65 | 59.25 | 238 | 66 |
100.00%
|
100.00%
|
| Primer 542 | Pectobacterium atrosepticum | ATGGCGTTACGGTCCGTT | ACAGCCACCAACGATGCA | 59.34 | 59.89 | 191 | 66 |
100.00%
|
100.00%
|
| Primer 543 | Pectobacterium atrosepticum | TGGCGAAAGAGCTGTGCA | TCGCGTTGGCAAAAGCCT | 59.89 | 60.90 | 288 | 66 |
100.00%
|
100.00%
|
| Primer 544 | Pectobacterium atrosepticum | TGATGCACCTGCGCTGTT | ACGCAGGTGCCGATCAAT | 60.28 | 59.73 | 189 | 66 |
100.00%
|
100.00%
|
| Primer 545 | Pectobacterium atrosepticum | AGCGCATCAATGTCGGGT | AGAGCAACGCAGGCTGTT | 59.73 | 59.89 | 211 | 66 |
100.00%
|
100.00%
|
| Primer 546 | Pectobacterium atrosepticum | GCGTTCTTGGTGGCAACTG | AACCTGCCAGTTGCCGTT | 60.01 | 60.12 | 232 | 66 |
100.00%
|
100.00%
|
| Primer 547 | Pectobacterium atrosepticum | CAGCGTTCTTGGTGGCAAC | AACCTGCCAGTTGCCGTT | 60.01 | 60.12 | 234 | 66 |
100.00%
|
100.00%
|
| Primer 548 | Pectobacterium atrosepticum | CGAACCGAGAAGAGCAGCA | TTGCAGCCCAACAGCTCA | 60.08 | 59.80 | 265 | 65 |
100.00%
|
100.00%
|
| Primer 549 | Pectobacterium atrosepticum | ACGCACGTAACGCCATGA | TCGGTGAATTCGCTGCGA | 60.05 | 59.74 | 259 | 65 |
100.00%
|
100.00%
|
| Primer 550 | Pectobacterium atrosepticum | TGCGGTGCTGCTGATTCA | TTCAGCAGCGCACGATCA | 59.97 | 60.05 | 186 | 65 |
100.00%
|
100.00%
|